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Rafael Lira

@BiophysLira

🇧🇷 - 🇩🇪 - 🇳🇱
Biophysicist and music enthusiast
Joined March 2021
715 Following 296 Followers 174 Posts

Latest posts 5

Rafael Lira @BiophysLira
The reconstitution of growth and division in synthetic cells faces many challenges. Here, we show one of the most efficient growth system ever described, followed by division, with lipid flip flop as a key regulator of membrane shape

biorxiv.org/content/10.110…
www.biorxiv.org Lipid flip flop regulates the shape of growing and dividing synthetic cells Cells grow their boundaries by incorporating newly synthesized lipids into their membranes as well as through fusion of intracellular vesicles. As these processes yield trans-bilayer imbalances in...
Rafael Lira @BiophysLira
Fusogenic liposomes are an excellent alternative to internalization-based particles for intracellular delivery of cargos. But they don't come without effects.
Here we show how
onlinelibrary.wiley.com/doi/full/10.10…
@univgroningen, @FLIM, @GUVs, @drugdelivery
onlinelibrary.wiley.com Membrane Fusion‐Based Drug Delivery Liposomes Transiently Modify the Material Properties of... The table of content summarizes the main development and findings of this study. Fusion of small liposomes with acceptor model or biomembranes modify the material properties of these membranes. In...
Rafael Lira @BiophysLira
Polymer droplets exhibit a set of material properties. Small polymers require high concentrations for phase separation. Ultralarge polymers achieve it at much lower concentrations. Are their properties characterized by polymer size, concentration, or both?
Here we address this:
Rafael Lira @BiophysLira
Liposomal drug-delivery vehicles that fuse with cells and directly and quickly deliver their cargos in the cytosol can be extremely efficient, but this efficiency may come with a price. Here we show how.

biorxiv.org/content/10.110…
www.biorxiv.org Membrane fusion-based drug delivery liposomes transiently modify the material properties of... Many drug targets are located in intracellular compartments of cells but they often remain inaccessible to standard imaging and therapeutic agents. To aid intracellular delivery, drug carrier...
2 replies 3 reposts 37 likes 1.8K views View on X
Rafael Lira @BiophysLira
FLIM is an excellent tool to probe the local microenvironment, requiring probes that sensitively report environmental properties. Here, we compare the performance of various reporters in sensing the environment of synthetic and living cells using FLIM.

cell.com/biophysj/fullt…
www.cell.com Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical proper... Sensing of the biophysical properties of membranes using molecular reporters has recently regained widespread attention. This was elicited by the development of new probes of exquisite optical...