David B Sauer

@DavidBSauer

Membrane Transporters, Ion Channels, Structural Biology, Bioinformatics... and other bits of fun

Purdue BS, UTSW PhD, NYUSoM PostDoc, Univ. of Oxford PI

New York, NY davidbsauer.com Joined February 2018

614 Following 1.2K Followers 718 Posts

Latest posts 4

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David B Sauer @DavidBSauer Mar 12

Step-by-step protocol to make stable cell lines for large-scale expression of muscle-type nAChR (and other multi-subunit targets)!

By the inexhaustible and tenacious @Anna_Liiiiiiii
in collab of @CMS_CDG_Oxford in @MRC_WIMM and @CmdOxford

cell.com/star-protocols…

www.cell.com Protocol for stable cell line production to express muscle-type nicotinic receptor The adult muscle-type nicotinic acetylcholine receptor (AChR) is essential for neuromuscular transmission but is difficult to produce due to the requirement for coordinated subunit assembly. Here, we...

David B Sauer @DavidBSauer Apr 24

In another case of transporters doing more than you expect: SPNS2 is a glucose importer, in addition to its S1P export activity!

Wonderful collaboration with Sarah Spiegel and colleagues at VCU School of Medicine, and the team at @CmdOxford

nature.com/articles/s4146…

www.nature.com SPNS2 exports sphingosine-1-phosphate and imports glucose Nature Communications - Spinster homolog 2 (SPNS2) exports sphingosine-1- 26 phosphate (S1P) out of cells. Here, the authors show that SPNS2 has antiporter-like activity, exporting S1P while...

David B Sauer @DavidBSauer Feb 18

Our study of the CAT1 amino acid transporter and viral receptor is now online in @NatureComms!

Led by the incredibly talented @mingda_ye, with colleagues from @CmdOxford, @OxfordStrubi, @CAMS_COI_Ox, @NDMOxford, and supported by @im2pact_project

nature.com/articles/s4146…

www.nature.com Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus... Nature Communications - Cationic amino acids are essential to protein synthesis and cellular signaling. Here, authors determine the structure of the cationic amino acid transporter 1 and determined...

David B Sauer @DavidBSauer Jun 25, 2025

Closing soon!

Please apply if you are interested in targeting membrane proteins with new therapeutics!

David B Sauer @DavidBSauer Jun 13, 2025

Join our group in the Centre for Medicines Discovery at Oxford Univ. @CmdOxford @UniofOxford !!!!

Happy to chat about any questions about the role and science.

Oxford will assist with a skilled worker visa.

Please RT!

my.corehr.com/pls/uoxrecruit…

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