Francis O'Reilly

@FJ_OReilly

Structural systems biology at the NCI/NIH. Views are my own.

Frederick, MD Joined July 2017

1.4K Following 767 Followers 746 Posts

Latest posts 5

PhIX-MS is out in Molecular Cell! We use rapid in situ photo-crosslinking to trap transient proteasome interactions, then combine crosslinking MS, cryo-EM and AlphaFold to define how they bind.

Paper: doi.org/10.1016/j.molc…
Preview: doi.org/10.1016/j.molc…

Francis O'Reilly @FJ_OReilly Jul 21, 2025

Time to get signed up for the next Symposium on Structural Proteomics! This time in beautiful Milan, 6th-8th October.
ssp2025.squarespace.com

Francis O'Reilly @FJ_OReilly Sep 11, 2024

New preprint! Apoptosis-inducing factor 1 (AIFM1) and Adenylate Kinase 2 (AK2) form a complex that links NADH-sensing to local ADP regeneration in the Mito intermembrane space - controlling substrate supply to the ATP synthase.
biorxiv.org/content/10.110…

www.biorxiv.org An NADH-controlled gatekeeper of ATP synthase ATP fuels crucial cellular processes and is obtained mostly by oxidative phosphorylation (OXPHOS) at the inner mitochondrial membrane. While significant progress has been made in mechanistic unders...

Francis O'Reilly @FJ_OReilly Sep 11, 2024

New preprint! We show that photo-crosslinking dramatically improves structural insights into protein complexes compared to traditional crosslinking MS methods.
biorxiv.org/content/10.110…

www.biorxiv.org Molecular insights into dynamic protein structures by high-contrast crosslinking mass spectrometry Proteins are comprised of structured domains and dynamic regions, and both are essential for biological function. However, studying dynamic regions is challenging using most structural biology...

Francis O'Reilly @FJ_OReilly Sep 11, 2024

There may be more eyes on hellsite tonight so I'll take the opportunity to tweet about our two recent preprints.