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Empty capsids are the tax on AAV manufacturing. So we made the capsid and its payload find each other: a TALE domain fused to VP2, its target sequence in the payload. Capsid filling up to ~3x, titres held. New in Molecular Therapy Advances: cell.com/molecular-ther…
www.cell.com VP2-TALE fusions increase AAV2 capsid filling with payload bearing cognate TALE target sequences as... Empty capsids dog AAV2 biomanufacturing. Nesbeth and colleagues genetically fused the AAV2 capsid protein, VP2, to a transcription activator-like effector (TALE) domain whilst in parallel inserting... 
