Twitxy Saved

Stefan Peidli

@SPeidli

German single-cell nerd
Heidelberg stefanpeidli.github.io Joined October 2019
273 Following 117 Followers 49 Posts

Latest posts 5

Pinned post

Stefan Peidli @SPeidli
Check out scPerturb, out now in Nature Methods!

Among other things: new datasets in our database, and a lot more information per dataset (e.g. mean No. of counts, cells per perturbation) in the interactive data table. You can find it on our website, link in thread!
Tessa Green @the_tessallator Jan 26, 2024
"scPerturb: harmonized single-cell perturbation data" is out now in Nature Methods!
View quoted post on X
1 replies 2 reposts 15 likes 1.4K views View on X
Stefan Peidli @SPeidli
Are you developing or benchmarking methods for single-cell perturbation data?
I've written a very simple example pipeline in snakemake that auto downloads all (/selected) datasets from scperturb and applies your custom script to each of them in parallel: github.com/stefanpeidli/s…
github.com GitHub - stefanpeidli/scperturb_snakemake_example: Example repository for analyzing many datasets... Example repository for analyzing many datasets at once from scperturb.org using snakemake - stefanpeidli/scperturb_snakemake_example
Stefan Peidli @SPeidli
Check out datavzrd, super useful tool for making tables pretty for showing on the web. I used it to make a simple metadata table for a database, see here: projects.sanderlab.org/scperturb/data…
#datavzrd, our tool for rendering modern interactive, visual, server-free, tabular scientific HTML reports with zero/low-code (datavzrd.github.io), now has a tutorial (1-2h): datavzrd.github.io/docs/tutorial.….
View quoted post on X
Stefan Peidli @SPeidli
Very unique data with mutliple doses and time points after infection. We had lots of opportunity for data analysis. And go check out the amazing diffusion map we got for the endothelials!
What drives severe COVID-19? In our new preprint, we explore this question using and interspecies comparison of lung single-cell RNA-seq data – summary in the thread below, lead by @SPeidli and Samantha Praktiknjo! … (1/7)
View quoted post on X
0 replies 3 reposts 8 likes 1.1K views View on X
Stefan Peidli @SPeidli
Cluster analysis is the first thing most PhD students do when working with scRNAseq data because it's simple and part of scanpy/seurat's tutorials. But often enough data does not actually allow clustering because it is too homogeneous (e.g. cell lines)!
Clustering algorithms report clusters even when none exist. In single-cell RNA-Seq pipelines, novel cell types are often identified by clustering algorithms. Expanding on Kimes et al.'s work, we introduce significance analysis for single-cell RNA-Seq data: nature.com/articles/s4159…
View quoted post on X