Mitch Guttman

@mitchguttman

Molecular biologist interested in non-coding RNAs, nuclear organization, and gene regulation. Professor at Caltech

Los Angeles, CA guttmanlab.caltech.edu Joined May 2015

222 Following 3.5K Followers 337 Posts

Latest posts 5

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Mitch Guttman @mitchguttman Nov 27, 2024

Gene regulation involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. Our paper in @NatureGenet describes ChIP-DIP, a method for genome-wide mapping of hundreds of DNA-protein interactions in a single experiment.

nature.com/articles/s4158…

www.nature.com ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene... Nature Genetics - ChIP-DIP (ChIP done in parallel) is a highly multiplex assay for protein–DNA binding, scalable to hundreds of proteins including modified histones, chromatin regulators and...

Mitch Guttman @mitchguttman Jul 24, 2025

Many proteins bind RNA, yet we still don’t know what RNAs most bind because methods map one RBP at a time. In @CellCellPress, with the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. sciencedirect.com/science/articl…

Mitch Guttman @mitchguttman Nov 15, 2024

A report made counterintuitive claims that PRC2 binds
→ more RNA than PTBP1 & hnRNPU
→ RNAs that do not exist in the cell

We found a simple explanation: the authors ignore most RNA in the sample, leading to inflated background & misleading conclusions.
biorxiv.org/content/10.110…

www.biorxiv.org Failing to account for RNA quantity inflates background and leads to the misleading appearance that... We recently published biochemical and quantitative evidence that challenges the widespread claims that PRC2 binds directly to many RNAs in vivo . A recent preprint performs a re-analysis of some of...

Mitch Guttman @mitchguttman May 8, 2024

What are the functional roles of 3D genome organization? Our paper in @Nature led by @_prashantbhat demonstrates how dynamic 3D genome organization around nuclear speckles plays a crucial role in regulating mRNA splicing efficiency. rdcu.be/dHkez

Mitch Guttman @mitchguttman Feb 22, 2024

PRC2 is reported to bind RNA. Yet, genetic experiments have raised serious questions about their functional relevance. Our paper revisits the biochemical evidence supporting widespread PRC2-RNA interactions and finds that many of these do not occur in vivo. tinyurl.com/mr3thkyx