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narf42

@narf42

Associate Professor for Molecular Evolution @univgroningen
; passionate scientist and insomniac. News, views & random things
Groningen, Nederland mayerlab.nl/index.html Joined June 2014
630 Following 1.4K Followers 2.1K Posts

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narf42 @narf42
🥳🥳🥳 Our study on engineering enzymes to break C–F bonds is now out in @angew_chem! Congrats to the brilliant & dedicated Suzanne Jansen for taking this first step toward the future bioremediation of #PFAS & other contaminating organofluorides!
onlinelibrary.wiley.com/doi/10.1002/an…
onlinelibrary.wiley.com Engineering Fluoroacetate Dehalogenase by Growth‐Based Selections on Non‐Natural Organofluorides We present a high-throughput selection system to engineer fluoroacetate dehalogenases (FAcDs). By challenging E. coli populations that produce diverse FAcD libraries to grow on non-natural organofl...
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narf42 @narf42
🚨New preprint! What a difference one C-F bond can make!🚨

Fluoroacetate dehalogenases (FAcDs) are remarkably good at breaking C-F bonds. But add just ONE more C-F bond to the substrate and suddenly the enzyme is inhibited.

So what’s going on? (1/4)
biorxiv.org/content/10.648…
www.biorxiv.org Substituting a conserved histidine alleviates difluoroacetate-dependent isoaspartate formation in a... Organofluorides are ubiquitous in modern society, but their persistence poses significant environmental and health risks. Fluoroacetate dehalogenases (FAcDs) are promising candidates for the biorem...
narf42 @narf42
🧵 New review out in Chemistry – A European Journal!

Hybrid macrocyclic peptides: combining biological selection with synthetic chemistry to discover next-generation therapeutics.

Why are hybrid macrocyclic peptides so exciting? 👇

(1/5)
…mistry-europe.onlinelibrary.wiley.com/doi/10.1002/ch…
chemistry-europe.onlinelibrary.wiley.com Hybrid Macrocyclic Peptides — Synthetic Strategies to Diversify and Cyclize Peptide Libraries in... Hybrid macrocyclic peptides unite genetically encoded peptide libraries with the structural complexity of synthetic small molecules. This Review critically analyzes phage-compatible cyclization and...
narf42 @narf42
With #PFAS and other organofluorides wreaking havoc, we set out to engineer natural defluorinases for man-made organofluorides. All credit goes to the amazing @suzanne__jansen, who has outdone herself in this project! (1/5)
biorxiv.org/content/10.110…
www.biorxiv.org Engineering fluoroacetate dehalogenase by growth-based selections to degrade non-natural organofl... The widespread use of organofluorides in modern society has inadvertently led to the bioaccumulation of harmful pollutants, most prominently per- and polyfluorinated alkyl substances (PFAS). In...