Roth_Lab

Transcription Factors are central to cellular identity and function. The human genome currently contains ~1600 of them. But could there be productive human transcription factors that have not evolved? Out today in Cell, a tremendously talented graduate student Oliver Takacsi-Nagy @Oli_TN, together with the @Satpathology Lab, determined to find out. (1/10)

authors.elsevier.com/sd/article/S00…

The size of new DNA sequences that can be integrated into the human genome is a foundational constraint for engineering and enhancing human cells. In a new collaborative study, we’ve now almost doubled the maximum size of DNA sequences that can be efficiently inserted into primary human cells.

biorxiv.org/content/10.648…

Genome wide perturb seq creates powerful perturbations x transcripts datasets. This unveils new biology - but also reveals new off-target effects. New work from @AustinMHartman, a massively talented PhD student in our lab, systematically identifies seed driven off-target effects in genome-wide perturb seq exps. (1 of 3) biorxiv.org/content/10.648…

Excited to present the first major work after starting our lab at Stanford and the Arc this year: CRISPR-All, a unified genetic perturbation language for programming any major type of genetic perturbation simultaneously, in any combination, at genome scale, in human cells.