Just published in @natBME: expansion revealing (ExR), a 20x #expansionmicroscopy method that evenly separates proteins from each other for better antibody labeling, and nanoprecise imaging, on ordinary microscopes. Link to PDF: rdcu.be/cUyAO
Ed Boyden
@eboyden3
Y. Eva Tan Professor in Neurotechnology, MIT. Mindspan Institute, co-founder, co-CEO. Neuroscientist, inventor, entrepreneur.
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Temporally multiplexed imaging (TMI) helps ordinary microscopes image many dynamic signals in a living cell. Different signals are associated with fluorophores with different clocklike properties. A movie of a cell is unmixed to see individual signals. biorxiv.org/content/10.110…
Seeing pictures of the GABA receptor, otoferlin, and calmodulin -- taken on an ordinary light microscope using ONE (one nanometer expansion microscopy) -- was thrilling.
The revolution will not be vitrified! Move over #cryoEM & #cryoET, welcome #ONE_Microscopy 😍biorxiv.org/cgi/content/sh… Amazing work from @AliHShaib and the Rizzoli lab, building on concepts from @eboyden3 and @HenriquesLab. With a #GABAA receptor teaser from @danielmihaylov7 😉View quoted post on X
“Expansion microscopy at one nanometer resolution” - so fun to see the shapes of proteins, on an ordinary light microscope!
Hello world, ONE is here... I am happy to share with you a new technology —termed #ONE_Microscopy, that allows a resolution approaching ~8 Å using conventional light microscopy.
@LabSauer @eboyden3 @RaduAricescu
Full story:
biorxiv.org/cgi/content/sh…View quoted post on X
#Expansionmicroscopy helps see heat shock proteins (Timmerman, Baldwin, and Benesch labs, biorxiv.org/content/10.110…), the pathogen Chlamydia trachomatis (Rudel lab, frontiersin.org/articles/10.33…), and a Drosophila larva (Preibisch lab, nature.com/articles/s4159…). From the last paper:
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