@namude
Luminal surface proteome of the brain vasculature uncovers blood-brain barrier regulators @ScienceMagazine:View quoted post on X
science.org/doi/10.1126/sc…
Big thanks to Zijian, Zuzhi, Yupu, Khanh, Yuxiang, and all collaborators to make this possible!
Our paper is now officially published in @NatureMedicine!View quoted post on X
Thrilled to share the final version online: nature.com/articles/s4159…
Thankful to my co-authors, collaborators, and our lab for the support.
@kaelin_lab @DrChoueiri @PitarresiLab @namude @DanaFarber
APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2 is a liability. We now report LaccID, a multicopper oxidase evolved from an ancestral fungal laccase that oxidizes aromatic substrates using non-toxic O2 instead of H2O2. We use LaccID for EM imaging of cultured cells and fly brains, and for proteomic mapping of the changing surface composition of T cells that engage with tumor cells via antigen-specific T cell receptors.View quoted post on X
www.mcponline.org From Fringe to the Mainstream: How ETD MS Brought O-GlcNAc to the Masses O-GlcNAcylation was identified in the 1980s by Torres and Hart and modifies thousands of cellular proteins, yet the regulatory role of O-GlcNAc is still poorly understood compared to the abundance of... Glad to share our latest study on the cell-surface proteome of human dendritic cells, by our awesome long-term collaborators Namrata and Charles @BroadProteomics and talented undergrad @Zuzhi_Jiang (soon at @UCSF tetrad for PhD).View quoted post on X
journals.aai.org/jimmunol/artic…
