Takeshi Uenaka

@onlytuenaka

🧠 Neurologist 🇯🇵 | Postdoc @WernigLab @Stanford 🧫 iPSC-derived models for studying neurodegeneration 🔬 Former @BrainResilience | Current @hdfcures Fellow

Joined March 2022

29 Following 37 Followers 55 Posts

Latest posts 5

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Takeshi Uenaka @onlytuenaka Feb 19

🧠A “stable” #iPSC transgene —
until differentiation.
Then it goes silent...

We turned that frustration into a solution.

Our @CellStemCell paper maps what actually keeps expression on👇

🔗Free access (50 days):
sciencedirect.com/science/authSh…
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Takeshi Uenaka @onlytuenaka Feb 24

Have you ever cried in the tissue culture room because your #iPSC transgene got silenced after differentiation?

I have. Many, many times.

So we built TK4, a PiggyBac vector designed to resist silencing.
Now available on @Addgene:
#239046 / #239047

Any feedback is welcome!

Takeshi Uenaka @onlytuenaka Feb 13

Thrilled to see our paper out in @CellStemCell!

Cell Stem Cell @CellStemCell Feb 13

Online Now! Prevention of transgene silencing during human pluripotent stem cell differentiation dlvr.it/TQxWrt #stemcells
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Takeshi Uenaka @onlytuenaka Feb 13

Our paper on preventing transgene silencing is now online in @CellStemCell!
🔗 Free access (50 days): sciencedirect.com/science/authSh…

Takeshi Uenaka @onlytuenaka Apr 15, 2025

🧠Have you ever cried when your #iPSC transgene vanished after differentiation?

Yeah, we’ve cried too, and built a solution.
Our PiggyBac vector keeps expression stable even after differentiation!

Check out our #preprint👇
🔗 biorxiv.org/content/10.110…

Supported by @cziscience

www.biorxiv.org Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing...