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Slavov Laboratory

@slavovLab

We seek principles in the coordination among protein synthesis, metabolism, cell growth and differentiation PI: @slavov_n Videos: youtube.slavovlab.net
Boston, MA slavovlab.net Joined July 2020
122 Following 3.1K Followers 623 Posts

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Slavov Laboratory @slavovLab
We report many proteins not predicted by the genetic code.

They are stable & abundant O( 10³ ) copies / cell.

Generative mechanisms include codon-anticodon mismatches & RNA modifications.

Their abundance depends on codon frequency & protein stability.

biorxiv.org/content/10.110…
www.biorxiv.org Alternate RNA decoding results in stable and abundant proteins in mammals Amino acid substitutions may substantially alter protein stability and function, but the con-tribution of substitutions arising from alternate translation (deviations from the genetic code) is...
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Slavov Laboratory @slavovLab
Protein functions depend on the full amino acid sequence, including isoforms, cleavage products, and modified residues that distinguish proteoform states.

To capture these modifications, we and @ParallelSqTech introduce multiDIA to increases proteome sequence coverage.
Prof. Nikolai Slavov Verified account @slavov_n Oct 20
Proteomics has long faced a choice: Go deep, or go fast.

Deep, high-confidence coverage of protein sequences has traditionally required extensive chromatographic fractionation, large sample inputs and data acquisition times too long to be practical for analyzing human cohort scale samples. Scalable data-independent acquisition (DIA) workflows handle many samples quickly, but detect less of each protein and with lower confidence.

Our new preprint introduces multiDIA: It gets more from each sample without fractionation.

1/

doi.org/10.64898/2026.…
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Slavov Laboratory @slavovLab
A very thoughtful and insightful discussion with @JShendure.

Most of it focuses on the development of DNA sequencing by synthesis, but the ideas and lessons from that experience are general and applicable to other technologies.

youtube.com/watch?v=tXrVFm…
www.youtube.com NHGRI's Oral History Collection: Interview with Jay Shendure Jay Shendure, M.D., Ph.D. is a Professor in the Department of Genom...