@RonZhu2015
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www.cell.com Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell... A dynamic atlas of gene regulation was generated by perturbing every expressed gene across 22 million primary human CD4+ T cells under resting conditions and following re-stimulation. The resulting... Together with Emma Dann, we are thrilled to present a massive new Perturb-seq atlas of 22M primary CD4+ T cells, from 4 donors, across 3 timepoints – the result of a decade-long collaboration between the Marson (@MarsonLab) and Pritchard (@jkpritch) labs.
🧵👇View quoted post on X
Excited to share a new preprint from the lab led by @LukeKoblan and @WilliamNColgan in which we describe our efforts to define a quantitative cell fate map of mouse embryogenesis! biorxiv.org/content/10.648…View quoted post on X
1/6 I was reanalyzing Perturb-seq data and stumbled into what I thought was a new TCR signaling regulator, only to realize it was an off-target effect. The guide ‘seed’ aligned near a canonical pathway member's TSS leading to off-target repression and association with the pathwayView quoted post on X
Excited to share VIPerturb-seq!View quoted post on X
New tech from my lab which aims to improve the cost, data quality, and efficiency of single-cell CRISPR screens so that they are accessible to any lab - even at genome-wide scale
Preprint and 🧵 (1/): biorxiv.org/content/10.648…
