Bintu Lab

@BintuLab

Single-cell chromatin and gene regulation dynamics for better cell engineering @ Stanford BioE

Stanford, CA bintulab.com Joined November 2018

155 Following 1.5K Followers 105 Posts

Latest posts 5

We used HT-recruit repeatedly to discover new protein domains involved in gene regulation and test many mutants. It can be used for domains associated with other functions (if you have a reporter). Now we published a detailed protocol on how to do it: nature.com/articles/s4159…

www.nature.com High-throughput measurements of protein domain functions using magnetic separation Nature Protocols - This Protocol presents a scalable magnetic separation-based method for screening large protein domain libraries in mammalian cells, providing a simple and quantitative...

Josh Tycko @JoshTycko Jun 30

We hope this protocol is useful for people doing large scale protein function work! nature.com/articles/s4159…

Led by @abbythurm from @BintuLab
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Bintu Lab @BintuLab Jul 22, 2024

Our first RNA-centric preprint is online! Congratulations to @abbythurm and the whole team - we're so excited to continue learning about effector domains in RBPs, and to hear your thoughts about what could come next!

Abby Thurm @abbythurm Jul 22, 2024

Do human RNA-binding proteins have modular regulatory domains that downregulate RNA lifetimes? Our preprint @BintuLab studying this question is now out on bioRxiv, where we indeed identify small domains in RBPs that potently induce RNA degradation! biorxiv.org/content/10.110… (1/9)
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Bintu Lab @BintuLab Jun 3, 2024

We are hiring a lab manager/research assistant! More details and how to apply here: bintulab.com/2024/06/01/hir…

bintulab.com Outdated: Hiring Lab Manager/Research Assistant An opening is available for a job (1-2 year commitment, 2 years preferred) as lab manager and research assistant in the Bintu Lab in the Department of Bioengineering at Stanford University. The Bin…

Bintu Lab @BintuLab Feb 24, 2024

A little late, but we're excited to share our preprint in collaboration with the Greenleaf lab that uses single-molecule footprinting at an engineered locus in mammalian cells to understand how TF occupancy dictates expression!

William J. Greenleaf @WJGreenleaf Feb 5, 2024

Our work using single-molecule chromatin configurations to link TF binding to gene expression is now out on bioRxiv! Amazing effort from @bgrdoughty, @MichaelaThinks, and @juliaschaepe, and an immensely rewarding collaboration with the @BintuLab: biorxiv.org/content/10.110… [1/n]
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Bintu Lab @BintuLab Oct 17, 2023

It's a double feature on epigenetic memory today! Check another amazing new preprint on single-cell chromatin compaction and memory establishment from wizard postdoc @fjmrt!

Taihei Fujimori @fjmrt Oct 11, 2023

New preprint! We studied how single-cell 3D chromatin structure changes under targeted epigenetic perturbations using multiplexed DNA FISH and synthetic biology tools, and found an intriguing relationship between chromatin compaction and epigenetic memory. biorxiv.org/content/10.110…
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