@josephmreplogle
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www.biorxiv.org Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells Perturb-seq is a powerful approach to systematically assess how genes and enhancers impact the molecular and cellular pathways of development and disease. However, technical challenges have limited... Modern GWAS can identify 1000s of hits but it can be hard to turn this into biological insight. What key cellular functions link genetic variation to disease?
I'm excited to present our new work combining associations + Perturb-seq to build interpretable causal graphs! A🧵View quoted post on X
Can we recreate the transcriptional states seen in single-cell atlas projects? In our new preprint we try to do this using large-scale Perturb-seq experiments. Along the way we discuss off-target effects of CRISPR epigenetic editors and identify drivers of key fibroblast states.View quoted post on X
